Which of the following is NOT listed as a laboratory test performed on blood collection in ACD tubes?

• A. Blood Bank studies
• B. HCA phenotyping
• C. DNA paternity testing
• D. ABO blood typing

Answer: (D)

When blood is collected in an ACD tube, the additive acts as an anticoagulant but also helps preserve nucleated cells and DNA, making the specimen suitable for genetic and immunogenetic testing. Because of this, tests that rely on DNA or viable cells—such as blood bank studies (which involve antigen testing and serology on preserved cells), HLA phenotyping, and DNA-based paternity testing—are typically performed on ACD-preserved samples.

ABO blood typing, on the other hand, is a serologic test that assesses red cell antigens using antibodies in serum or plasma and is usually done with specimens collected in other tube types (like EDTA or serum tubes). Therefore, ABO typing is not generally listed as a test performed on blood collected in ACD tubes, making it the exception.